bkdAA [2018-02-12T13:37:07.000Z]

2-oxoisovalerate dehydrogenase (E1 alpha subunit)

Locus: BSU24050

Isoelectric point: 4.78

Molecular weight: 36.18 kDa

Protein length: 330 aa

Gene length: 990 bp

Function: utilization of branched-chain keto acids

Product: 2-oxoisovalerate dehydrogenase (E1 alpha subunit)

Essential: no

E.C.: 1.2.4.4

Synonyms: bfmBAA, bfmB1a, bkd

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids

Gene

Coordinates: 2,499,090 → 2,500,082

The protein

Catalyzed reaction/ biological activity

• 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂ (according to Swiss-Prot)

Protein family

• BCKDHA family (according to Swiss-Prot)

Paralogous protein(s)

• AcoA, PdhA

Structure

• 1UMB (from Thermus thermophilus, 42% identity, 57% similarity) PubMed

Localization

• Membrane-proximal (Spotty) PubMed

Expression and Regulation

Operons

• Genes: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
  Description: PubMed

  Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

  Regulatory mechanism

  • BkdR: activation, PubMed, in BkdR regulon
  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • induced in the presence of isoleucine or valine (BkdR) PubMed

Biological materials

Mutant

• BKE24050 (ΔbkdAA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAG
• BKK24050 (ΔbkdAA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAG

References

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